FRTL-5 Rat Thyroid Cells Release Thyroglobulin Sequestered in Exosomes: A Possible Novel Mechanism for Thyroglobulin Processing in the Thyroid

Figure 2

SDS-PAGE and Western blot with anti-Tg of samples from different stages of exosome isolation by ultracentrifugation: 17,000 ×g pellet and supernatant, 200,000 ×g supernatant, and 200,000 ×g (exosome pellet) from 6H medium collected from a confluent culture of FRTL-5 cells (lanes 1–4) and fresh 5H medium with 1 mg/mL bovine Tg added (lanes 5–8). Western blots were treated with a monoclonal anti-human-Tg antibody and developed using SuperSignal West Pico. “Exos” = exosomes (i.e., 200,000 ×g pellet).