Human Liver Cells Expressing Albumin and Mesenchymal Characteristics Give Rise to Insulin-Producing Cells
Figure 2
Primary adult human liver cell cultures express hepatic and mesenchymal stem-cell markers and differentiate along adipogenic and osteogenic lineages. (a) Adult human liver cells (P1–P7, ) were characterized for the expression of specific surface and intracellular markers of hepatic, hepatic progenitor, mesenchymal, and hematopoietic lineages by flow cytometry. (b–d) Double immunofluorescent staining of human liver cells (P7) for albumin and CD90. Nuclei were stained by DAPI (blue). Original magnification . (e) Liver cells at P4 were induced to differentiate toward the adipogenic and (g) osteogenic lineages using the specific differentiation cocktails for 21 days in culture. Control cells in regular cell culture media are shown in (f) and (h). Samples were fixed and stained with Oil Red (adipogenic lipids, (e), (f) or Alizarin (osteogenic calcium, (g), (h)). Original magnification .
