Human Liver Cells Expressing Albumin and Mesenchymal Characteristics Give Rise to Insulin-Producing Cells
Figure 5
Ectopic PDX-1 expression induced pancreatic differentiation mainly in eGFP positive liver cells. eGFP-positive cells and DsRed2-positive cells were treated with Ad-PDX-1 and soluble factors for 5 days. Quantitative RT-PCR analysis of (a) pancreatic hormones (INS, GG, and SST), and (b) β-cell specific genes (GK, GLUT2, and PC2) in eGFP-positive cells and DsRed2-positive cells ( p7–9). The results are normalized to β-actin expression within the same cDNA sample and presented as the relative levels of the mean ± standard deviation of PDX-1 treated cells versus control Ad-β-gal treated cells. *. (c–h) Double immunofluorescence analysis of GFP with insulin in Ad-PDX-1 treated eGFP-positive liver cells. Original magnification (c–e) and (f–h). Five hundred cells were analyzed under the fluorescent microscope for eGFP, DsRed2, or insulin in three different cultures.
