About this Journal Submit a Manuscript Table of Contents
Journal of Transplantation
Volume 2012 (2012), Article ID 127215, 6 pages
http://dx.doi.org/10.1155/2012/127215
Research Article

A Knotless Technique for Kidney Transplantation in the Mouse

1Division of Nephrology, Hannover Medical School, 30625 Hannover, Germany
2The Transplantation Center, Affiliated Hospital, Zunyi Medical College, Zunyi 563003, China
3Division of Developmental Biology, Cincinnati Children's Hospital Research Foundation, Cincinnati, OH 45229, USA
4Division of Nephrology and Immunology, RWTH University of Aachen, 52062 Aachen, Germany
5Phenos GmbH, 30625 Hannover, Germany

Received 24 March 2012; Revised 29 May 2012; Accepted 31 May 2012

Academic Editor: Wojciech Rowiński

Copyright © 2012 Song Rong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mouse models of kidney transplantation are important to study molecular mechanisms of organ transplant rejection as well as to develop new therapeutic strategies aimed at improving allograft survival. However, the surgical technique necessary to result in a viable allograft has traditionally proven to be complex and very demanding. Here, we introduce a new, simple, and rapid knotless technique for vessel anastomosis wherein the last stitch of the anastomosis is not tied to the short end of the upper tie as in the classical approach but is left free. This is a critical difference in that it allows the size of the anastomosis to be increased or decreased after graft reperfusion in order to avoid stenosis or bleeding, respectively. We compared the outcome of this new knotless technique ( 𝑛 = 1 7 5 ) with the classical approach ( 𝑛 = 1 2 2 ) in terms of local thrombosis or bleeding, time for anastomosis, and survival rates. By this modification of the suture technique, local thrombosis was significantly reduced (1.1% versus 6.6%), anastomosis time was less, and highly reproducible kidney graft survival was achieved (95% versus 84% with the classical approach). We believe that this knotless technique is easy to learn and will improve the success rates in the technically demanding model of mouse kidney transplantation.