A Knotless Technique for Kidney Transplantation in the Mouse
Figure 2
Arterial anastomosis ((a) and (b)) was performed in an end-to-side manner between the donor renal artery and recipient aorta. For ureteral anastomosis ((c)–(e)), the free end of the ureter was stripped of fat and introduced into the needle lumen with fine forceps, and the needle was gently withdrawn from the bladder, with the ureter accompanying it (c). Once it had exited the lower right bladder wall puncture site, the free end of the ureter was immediately clipped with a microvascular clamp, to avoid its retraction into the bladder (d). The ureter was anastomosed and the puncture wound in the bladder repaired (e). Situation after transplantation showing position of the transplanted kidney relative to the nephrectomy site and the anastomoses of the major blood vessels (f).
