Peripheral Regulatory Cells Immunophenotyping in Kidney Transplant Recipients with Different Clinical Profiles: A Cross-Sectional Study

Figure 3

Percentage of immature/transitional IL-10-producing B peripheral cell subtype in KTR. (a) Representative contour plot of CD19⁺ B cells from a patient. An electronic gate was made for CD38^hi cells. (b) From the gate 𝑎 CD19⁺⁄CD38^hi⁄CD24^hi cells were determined. (c) From the gate 𝑏 CD19⁺/CD38^hi/CD24^hi/CD5⁺ were defined and an electronic gate was made for positive cells. From the latter CD19⁺/CD38^hi/CD24^hi/CD5⁺/IL-10⁺ cells were determined in (d) a healthy donor (HD), (e) a chronic graft dysfunction (CGD) patient, and (f) an excellent long-term graft function under immunosuppression (ELTGF) patient. (g) Percentage of homing receptor-expressing Breg subtype in KTR. An electronic gate was made for CD38^hi cells. (h) From the gate 𝑔 CD19⁺/CD38^hi/CD24^hi cells were determined. (i) From the gate ℎ CD19⁺/CD38^hi/CD24^hi/CXCR7⁺ were defined. From the latter CD19⁺/CD38^hi/CD24^hi/CXCR7⁺/IL-10⁺ cells were determined in (j) a HD, (k) a CGD, and (l) an ELTGF. Bar graphs show percentage of (m) CD5⁺, (n) CD10⁺, (o) CD20⁺, (p) CD27⁺, (q) CXCR4⁺, and (r) CD19⁺/CD38^hi/CD24^hi/CXCR7⁺/IL-10⁺-producing B peripheral cells. A total of 30,000–50,000 events were recorded for each sample before any gate setting and analyzed with the CellQuest Pro software (BD Biosciences). Results are expressed as median, 10th, 25th, 75th, and 90th percentiles.

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