Peripheral Regulatory Cells Immunophenotyping in Kidney Transplant Recipients with Different Clinical Profiles: A Cross-Sectional Study

Figure 4

Percentage of IDO-and Foxp3-expressing peripheral blood cells in KTR. (a) Representative contour plot from a patient. An electronic gate was made for CD25^hi cells. (b) From the gate 𝑎, CD4⁺/CD25^hi were determined, and an electronic gate was made for double positive cells. From the latter, CD4⁺/CD25^hi/Foxp3⁺ cells were defined in (c) a healthy donor (HD), (d) a chronic graft dysfunction (CGD), and (k) an excellent long-term graft function under immunosuppression (ELTGF) patient. (f) An electronic gate was made for CCR6⁺ cells. (g) From the gate 𝑓, CCR6⁺/CD123⁺ cells were determined, and an electronic gate was made for double positive cells. From the latter, CCR6⁺/CD123⁺/IDO⁺ cells were defined in (h) a HD, (i) a CGD patient, and (j) an ELTGF patient. (k, l) Control of FITC-conjugated-rabbit antisheep specificity staining. Percentage of (m) CCR6⁺/CD123^hi/IDO⁺, (n) CD4⁺/CD25^hi/Foxp3⁺, and (o) CD8⁺/CD28⁻/Foxp3⁺ peripheral blood cells. A total of 50,000 events were recorded for each sample before any gate setting and analyzed with the CellQuestPro software (BD Biosciences). Results are expressed as median, 10th, 25th, 75th, and 90th percentiles.

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