Isolation of Human Islets for Autologous Islet Transplantation in Children and Adolescents with Chronic Pancreatitis

Figure 5

The pancreatic organ of young patients is subjected first to a stationary digestion characterized by prolonged infusion of prewarmed collagenase-neutral protease solution. Mechanical shaking is carried out after stationary digestion and limited in time to avoid excessive breakage of islets embedded in exocrine tissue.