Inflammation-Mediated Regulation of MicroRNA Expression in Transplanted Pancreatic Islets

Table 1

miRNAs expressed in islets treated in vitro with a cocktail of IL-1β, IFN-γ, and TNF-α were identified by SAM of global miRNAs. The score (𝑑) represents value of the T-statistic; a higher score means a greater difference between the two groups. 𝑞-values correspond to the 𝑃 values adapted to the analysis of a large number of genes. FI and SD are fold increase and standard deviation of hybridization values (cytokine versus control), respectively.

Upregulated miRNAs	(𝑑)	𝑞 (%)	FI	SD		miRNA	(𝑑)	𝑞 (%)	FI	SD
miR-143	6.7	0	7.5	3.5		miR-27a	4.5	0	6.2	4.2
miR-30e	6.0	0	15.3	11.4		let-7a	4.4	0	8.4	7.1
miR-182	6.0	0	9.1	4.8		miR-375	4.4	0	7.8	6.4
miR-96	6.0	0	13.6	9.4		miR-30a	4.4	0	5.3	3.3
miR-141	5.9	0	13.2	10.6		miR-19b	4.3	0	6.6	4.5
miR-24	5.7	0	9.2	6.2		let-7f	4.3	0	19.8	25.4
miR-29b	5.5	0	12.7	11.2		miR-16	4.1	0	4.7	2.9
miR-212	5.5	0	9.8	7.0		miR-29c	4.1	0	10.6	10.2
miR-7a	5.2	0	10.7	8.6		miR-103	4.1	0	3.0	0.9
miR-19a	5.2	0	3.9	1.3		miR-148b-3p	4.0	0	7.1	6.1
let-7i	5.2	0	13.7	9.6		miR-30d	3.9	0	4.1	2.3
miR-153	5.1	0	25.0	27.8		miR-301a	3.9	0	22.3	26.5
miR-22	5.1	0	4.7	1.9		let-7c	3.9	0	5.1	3.5
miR-27b	5.0	0	8.1	5.7		miR-23b	3.8	0	4.3	2.5
miR-21	5.0	0	10.2	6.5		miR-29a	3.7	0	5.8	4.5
miR-30b-5p	5.0	0	6.6	4.2		miR-23a	3.6	0	4.3	2.6
let-7d	4.9	0	9.6	7.7		miR-204	3.6	0	7.4	8.2
miR-30c	4.8	0	6.5	4.0		miR-541	2.9	0	13.1	16.0
miR-200a	4.8	0	13.0	11.5		miR-99b	2.9	0	13.1	1.3
miR-207	4.8	0	4.6	2.2		rno-let-7b	2.8	0	3.6	2.3
miR-183	4.8	0	4.7	2.0		miR-125b-5p	2.7	0	3.0	1.7
miR-127	4.8	0	7.6	3.8		miR-17/17-5p	2.7	0	9.9	14.2
miR-107	4.7	0	6.0	3.7		miR-194	2.5	0	2.9	1.8
miR-335	4.7	0	2.8	0.7		miR-200c	2.4	0	2.6	1.4
miR-101a	4.7	0	3.9	2.7		miR-125a-5p	2.4	0	2.6	1.5
miR-26a	4.7	0	6.5	4.5		miR-200b	2.3	0	2.7	1.5
miR-98	4.6	0	9.3	7.8		miR-340-5p	2.2	0	2.3	1.1
miR-7b	4.6	0	14.0	13.4		let-7e	2.0	0	2.3	1.1
miR-126	4.5	0	4.5	2.2		miR-337	2.0	0	2.0	2.3
miR-106b	4.5	0	4.0	1.5
Downregulated miRNAs	(𝑑)	𝑞 (%)	FI	SD
miR-185	−2.8	0	4.0	3.3
miR-129	−3.0	0	2.8	1.5
miR-503	−3.2	0	4.1	3.2
miR-370	−3.4	0	3.5	2.1
miR-206	−4.3	0	2.6	0.7