Medical Gases: A Novel Strategy for Attenuating Ischemia—Reperfusion Injury in Organ Transplantation?
Figure 3
iNO decreases reperfusion-dependent hepatic cell death. (a) Histopathologic scoring of hepatic tissue samples before (white bars) and 1 hour after reperfusion (black bars). -values represent significance calculated by paired test. (b) Representative H&E-stained sections indicating increased injury in LB2. Original magnification ×25. The circled area is shown at a higher magnification (×100) in the inset and shows increased PMN infiltration adjacent to the hepatic vein (zone 3). (c) Representative fluorescence micrographs showing changes in TUNEL-positive cells (green); blue staining: DAPI. Original magnification, ×25. (d) Paired changes in TUNEL-positive objects in liver biopsies before (LB1) and 1 hour after reperfusion (LB2). values represent significance calculated by paired -test. (e) Average reperfusion-dependent increases in TUNEL-positive objects. * relative to placebo [17].
