Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin

Table 1

Number of islets obtained with and without BSA.

				Number of Islets/treatment group
Strain	Age (wks)	Sex	𝑛/group	With BSA SD	Without BSA SD
C57Bl/6	10	M	2	450±18.2	335±20.0
B6/129	19	F	2	516±27.4	382±11.3
BALB/c	8	M	2	436±27.2	304±13.6
NOD	8	F	3	625±17.6	245±6.0
NOD	9	F	4	685±15.5	540±12.1
C57Bl/10	10	F	2	330±10.6	275±11.9
C57Bl/10	18	F	3	595±30.5	436±12.5
FVB	20	F	3	940±25.2	690±19.7
		Totals	21	4577±119^∗	3207±97^∗

Islets were isolated by first distending the pancreas with collagenase solution (1.95 mg/mL Type V). The tissue was then digested and separated into 2 equal portions. One portion was processed with BSA in the washes throughout the isolation process and the other without BSA in the washes. Several strains of mice were used and their age in weeks, sex and number of mice in each treatment group (n/group) are indicated.
* P = 0.004.