Sirolimus Enhances Cyclosporine A-Induced Cytotoxicity in Human Renal Glomerular Mesangial Cells

<table>The MEK inhibitor, U0126, blocked CsA- and SRL-induced ERK 1/2 activity in HMCs. HMCs were grown to confluency and treated with vehicle, 4.2 <i>μ</i>M CsA, 1 <i>μ</i>M SRL, or CsA/SRL 4.2 <i>μ</i>M CsA + 1 <i>μ</i>M SRL in the presence or absence of 10 <i>μ</i>M UO126 for up to 48 hours. Phosphorylated ERK 1/2 MAPK or whole-cell ERK 1/2 MAPK was probed by Western blotting. Representative blots from one of three independent experiments are shown.</table>

Journal of Transplantation

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Figure 4

Figure 4: Sirolimus Enhances Cyclosporine A-Induced Cytotoxicity in Human Renal Glomerular Mesangial Cells 