Comparison of Oral Tolerance to ApoB and HSP60 Peptides in Preventing Atherosclerosis Lesion Formation in Apob48−/Ldlr− Mice
Figure 4
Oral tolerance to ApoB and HSP60 reduces atherosclerosis development and lipid accumulation in lesion. (a) Representative photomicrographs of aortic sinus plaque area stained with EVG (hearts were sectioned at the end of the study) in control, ApoB- and Hsp60-treated mice, respectively. Scale bar represents 200 μm (). (b) Representative photomicrographs of aortic sinus plaque area stained with Oil red O (ORO) (hearts were sectioned at the end of the study) in control, ApoB- and Hsp60-treated mice, respectively. Scale bar represents 200 μm (). (c) Plaque area in the aortic sinus in control, ApoB- and Hsp60-treated mice, respectively. (d) The lipid content in the lesion was detected by staining with Oil red O (ORO). Percentage of ORO positive area in the lesion in control, ApoB- and Hsp60-treated mice, respectively. (e) Concentrations of cholesterol and triglycerides were determined by Siemens Dimension Flex reagent cartridge (Siemens Healthcare Diagnostics Ltd, UK) using the Cobas Fara II Clinical Chemistry auto analyzer (F. Hoffman La Roche Ltd., Basel, Switzerland) as shown in the table. , , .
