Comparison of the Effect of Heterologous and Homologous Seminal Plasma on Motility and Chromatin Integrity of Stallion Spermatozoa Selected by Single Layer Centrifugation

Table 2

Effects of homologous or heterologous seminal plasma on stallion sperm chromatin integrity.

Treatment	Single layer centrifugation	Homologous SP	Heterologous SP
%DFI	6.3 ± 3.7^ab	10.6 ± 3.4^a	11.7 ± 4.0^b
Mean_DFI	292 ± 5.6^a	295 ± 8.1	300 ± 10.4^a
SD_DFI	54.5 ± 13.2	57.0 ± 11.0	59.0 ± 11.6

%DFI = DNA fragmentation index, Mean_DFI = mean fluorescence and SD-DFI standard deviation of fluorescence. SP = Seminal plasma. Seminal plasma was added to SLC samples at 0 h followed by storage at 6°C for 24 h before snap-freezing aliquots for SCSA. Same superscript within a row denotes statistical significance: ^a; ^b.