Real-Time RT-PCR for the Detection of Lyssavirus Species

Figure 1

Amplification of a CVS-11 serial dilution using hnRT-PCR. Ethidium bromide stained gel after amplification of the CVS-11 strain using dilutions from 10⁻¹ to 10⁻⁸ (A–H). The amplification product of 582 bp is clearly visible up to a dilution of 10⁻⁵ (lane E). Ladder = standard for determination of amplification product size.