Real-Time RT-PCR for the Detection of Lyssavirus Species

Table 1

Rabies viruses used.

Species	Designation	Host species	Origin, year of isolation/receipt^a	Material	Accession number^b
EBLV-1	Bat Stade	Bat (E. serotinus)	Germany (Stade), 1970	Mouse brain suspension	KF831524/KF831550
EBLV-1	Bat Hamburg RV 9	Bat (E. serotinus)	Germany (Hamburg), 1968	Mouse brain suspension	KF831526/KF831552
EBLV-2	Bat Finland RV 8	Human, bat exposure	Finland, 1986	Mouse brain suspension	KF831528/KF831553
EBLV-1	Bat Holland RV 31	Bat	Netherlands, 1988^a	Mouse brain suspension	KF831525/KF831551
RABV	Dog Tunisia	Dog	Tunisia, 1986	BHK-21 cell culture supernatant	KF831519/KF831545
EBLV-1	Bat Spain RV 119	Bat	Spain, 1989^a	BHK-21 cell culture supernatant	KF831527
RABV	Bat Florida	Yellow bat	USA (Florida), 1986^a	BHK-21 cell culture supernatant	KF831522/KF831548
EBLV-1	Bat Denmark	Bat (E. serotinus)	Denmark, 1986^a	BHK-21 cell culture supernatant	KF831523/KF831549
RABV	Raccoon dog Poland	Raccoon dog	Poland, 1985	Mouse brain suspension	KF831518/KF831544
RABV	Raccoon Florida	Raccoon	USA (Florida), 1986	BHK-21 cell culture supernatant	KF831521/KF831547
RABV	Bat Chile RV 108	Bat	Chile, 1988^a	Mouse brain suspension	KF831520/KF831546
RABV	Jackal Zimbabwe	Jackal	Zimbabwe, 1990^a	Mouse brain suspension	KF831529/KF831554
RABV	Cattle Zimbabwe	Cattle	Zimbabwe, 1990^a	Mouse brain suspension	KF831555
RABV	Canada Red Fox	Red Fox	Canada (Ontario), 1986^a	Mouse brain suspension	KF831530/KF831556
RABV	LEP	Human	USA (Georgia), 1939	BHK-21 cell culture supernatant	KF831531/KF831557
RABV	HEP	Human	USA, 1939	BHK-21 cell culture supernatant	KF831532/KF831568
RABV	Dog Nepal Nr. 96	Dog	Nepal, 1989^a	BHK-21 cell culture supernatant	KF831534/KF831559
RABV	Sri Lanka dog 121	Dog	Sri Lanka, 1986	BHK-21 cell culture supernatant	KF831535/KF831572
RABV	Eurofox 912/87	Fox	Switzerland, 1987	BHK-21 cell culture supernatant	KF831538/KF831562
DUVV	Duvenhage RV6	Human	South Africa, 1988^a	BHK-21 cell culture supernatant	KF831533/KF831558
RABV	NYC 58	Laboratory strain	USA, 1987^a	Mouse brain suspension	KF831539/KF831563
RABV	Dog Lima	Dog	Peru (Lima), 1985^a	Mouse brain suspension	KF831540/KF831564
EBLV-1	Bat Bremerhaven RV11	Bat	Germany (Bremerhaven), 1989^a	BHK-21 cell culture supernatant	KF831541/KF831565
EBLV-1	Bat B24	Bat	Europe, 1986^a	Mouse brain suspension	KF831536/KF831560
RABV	Skunk Canada	Striped skunk	Canada (Ontario), 1986	BHK-21 cell culture supernatant	KF831537/KF831561
RABV	SAD Bern	ERA*	USA, 1935 (received 1976)	BHK-21 cell culture supernatant	KF831542/KF831566
RABV	CSSR/A-virus	Rodent, laboratory strain	CSSR (Prague), 1987^a	BHK-21 cell culture supernatant	KF831543/KF831567
RABV	Challenge virus standard (CVS-11) ATCC VR 959	Laboratory strain	France (CNEVA), 1995^a	BHK-21 cell culture supernatant	GU992321
EBLV-2	TW 1814/92	Bat (M. daubentonii)	Switzerland (Plaffeien), 1992	Na 42/13 cell culture supernatant	KF831569
EBLV-2	TW 1392/93	Bat (M. daubentonii)	Switzerland (Versoix), 1993	Na 42/13 cell culture supernatant	KF831570
EBLV-2	TW 118/02	Bat (M. daubentonii)	Switzerland (Geneva), 2002	Na 42/13 cell culture supernatant	KF831571

Lyssaviruses of 4 different species were tested.
^aYear of receipt at the Swiss rabies center.
^bAccession numbers of 220 bp and 543 bp N fragments, respectively, sequenced in this work.
*Reference [36].