Research Article
Real-Time RT-PCR for the Detection of Lyssavirus Species
Table 3
Serial dilutions of the CVS-11 plasmid in real-time PCR (NDWD).
| | Dilution | Copy numbers/2 L | CT-value 1 | CT-value 2 | CT-value 3 | Mean ± SD | CV (%) |
| | 10−1 | 106 | 22.6 | 22.0 | 22.4 | 22.3 ± 0.31 | 1.37 | | 10−2 | 105 | 25.1 | 25.2 | 25.3 | 25.2 ± 0.10 | 0.40 | | 10−3 | 104 | 29.1 | 28.8 | 28.7 | 28.9 ± 0.21 | 0.72 | | 10−4 | 103 | 32.9 | 32.9 | 32.8 | 32.9 ± 0.06 | 0.18 | | 10−5 | 102 | 36.4 | 36.2 | 35.9 | 36.2 ± 0.25 | 0.70 | | 10−6 | 101 | 47.5 | 38.6 | 39.0 | 41.7 ± 5.03 | 12.05 | | 10−7 | 100 | — | — | — | — | — |
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SD: standard deviation; CV: coefficient of variation.
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