Real-Time RT-PCR for the Detection of Lyssavirus Species

Table 4

PCR-results of rabies viruses tested.

					Real-time RT-PCR (NDWD)
Sample		Species	hnRT-PCR		CT-value^a
				FAM (GT1)	YY (GT5)	Cy5 (GT6)
1	Bat Stade (1970)	EBLV-1	+		14.7	17.3
2	Bat Hamburg RV 9 (1968)	EBLV-1	+	20.2	14.8	16.4
3	Bat Finland RV 8 (1986)	EBLV-2	+			15.1
4	Bat Holland RV 31	EBLV-1	+	20.2	14.7	16.8
5	Dog Tunisia (1986)	RABV	+	24.6	27.2	33.2
6	Bat Spain RV 119	EBLV-1	+	33.4	27.6	31.6
7	Bat Florida	RABV	+	24.2	41.6
8	Bat Denmark	EBLV-1	+	26.5	21.1	22.4
9	Raccoon dog Poland (1985)	RABV	+	16.1		15.0
10	Raccoon Florida (1986)	RABV	+	25.8
11	Bat Chile RV 108	RABV	+	14.4
12	Jackal Zimbabwe	RABV	+	14.6
13	Cattle Zimbabwe	RABV	+	18.0
14	Canada Red Fox	RABV	+	17.9
15	LEP (Flury, 1939)	RABV	+	17.7
16	HEP (Flury)	RABV	+	28.5
17	Dog Nepal Nr. 96	RABV	+	21.7
18	Sri Lanka dog 121 (1986)	RABV	+	23.2
19	Eurofox 912/87	RABV	+	28.3
20	Duvenhage RV6	DUVV	+			27.0
21	NYC 58	RABV	+	16.5
22	Dog Lima	RABV	+	20.0
23	Bat Bremerhaven RV11	EBLV-1	+		27.0
24	Bat B24	EBLV-1	+		16.9	20.6
25	Skunk Canada	RABV	+	23.2
26	SAD Bern, 1935	RABV	+	18.8
27	CSSR/A-virus	RABV	+	18.5
28	Challenge virus standard (CVS-11) ATCC VR 959	RABV	+	19.8
29	TW 1814/92	EBLV-2	+
30	TW 1392/93	EBLV-2	+
31	TW 118/02	EBLV-2	+

Only for samples with positive reaction.
FAM: 6-carboxyfluorescein reporter dye, to detect RABV (GT1); YY: Yakima Yellow, to detect EBLV-1 (GT5); Cy5: Cyanine 5, to detect EBLV-2 (GT6); ND: not done.