Journal of Veterinary Medicine The latest articles from Hindawi Publishing Corporation © 2014 , Hindawi Publishing Corporation . All rights reserved. Comparison of the Effect of Heterologous and Homologous Seminal Plasma on Motility and Chromatin Integrity of Stallion Spermatozoa Selected by Single Layer Centrifugation Wed, 20 Aug 2014 06:00:00 +0000 The effect on sperm motility and chromatin integrity of adding homologous or heterologous equine seminal plasma (SP) to fresh stallion spermatozoa selected by single layer centrifugation (SLC) was studied. No statistical difference in mean progressive motility was seen after adding SP at time 0 h, although there were differences for individual stallions. The proportion of spermatozoa with high velocity was increased compared to untreated SLC-selected spermatozoa (), with significant differences between individuals (). When the SLC samples were stored for 24 h before adding SP, a significant increase in mean progressive motility was seen for SLC + homologous SP () and for SLC + heterologous SP (). Whether homologous SP or heterologous SP had a greater effect on progressive motility depended on the individual. Adding either type of SP caused a significant increase in chromatin damage compared to SLC after storage for 24 h (homologous SP, ; heterologous SP, ). These preliminary data showed that storage of SLC-spermatozoa mixed with SP should be avoided because of the risk of increased chromatin damage. If SP is to be added to take advantage of a transient increase in progressive motility for a particular individual stallion, different combinations of SP and spermatozoa should be tested first to optimize the effect. J. M. Morrell and A. Johannisson Copyright © 2014 J. M. Morrell and A. Johannisson. All rights reserved. Hepatic Necrosis and Degenerative Myopathy Associated with Cassava Feeding in Pigs Tue, 12 Aug 2014 12:55:39 +0000 Forty-three deaths were recorded among pigs fed boiled cassava meal at a private piggery over a period of two years. There were signs of sudden death in some cases with blood exuding from the external nares, vomiting, muscular weakness and pain or reluctance to move, emaciation, and stunted growth. The necropsy lesions included skeletal and cardiac muscle degeneration and necrosis, icterus, hepatic necrosis, and oedema of the dependent parts. The deaths and clinical signs are thought to be due to a non cyanide toxic principle in cassava, possibly the coumarins (scopoletin), which is found in high levels in cassava diet even after heat treatment. Therefore, the use of proper processing technology to obtain cassava products of high quality is recommended. Gloria Daniel-Igwe Copyright © 2014 Gloria Daniel-Igwe. All rights reserved. Lactation Curve Pattern and Prediction of Milk Production Performance in Crossbred Cows Sun, 13 Jul 2014 10:05:40 +0000 Data pertaining to 11728 test-day daily milk yields of normal and mastitis Karan Fries cows were collected from the institute herd and divided as mastitis and nonmastitis and parity-wise. The data of lactation curves of the normal and mastitis crossbred cows was analyzed using gamma type function. FTDMY in normal and mastitis cows showed an increasing trend from TD-1 to TD-4 and a gradual decrease thereafter until the end of lactation (TD-21) in different parities. The FTDMY was maximum (peak yield) in the fourth parity. Parity-wise lactation curve revealed a decrease in persistency, steeper decline in descending slope (c), and steeper increase in ascending slope (b) from 1st to 5th and above parity. The higher coefficient of determination and lower root mean square error (RMSE) indicated goodness and accuracy of the model for the prediction of milk prediction performance under field conditions. Clinical mastitis resulted in a significantly higher loss of milk yield . The FTDMY was maximum in the fourth parity in comparison to the rest of parity. It is demonstrated that gamma type function can give the best fit lactation curve in normal and mastitis infected crossbred cows. Suresh Jingar, R. K. Mehla, Mahendra Singh, and A. K. Roy Copyright © 2014 Suresh Jingar et al. All rights reserved. Comparative Pharmacokinetics of Cefquinome (Cobactan 2.5%) following Repeated Intramuscular Administrations in Sheep and Goats Mon, 19 May 2014 12:10:26 +0000 The comparative pharmacokinetic profile of cefquinome was studied in sheep and goats following repeated intramuscular (IM) administrations of 2 mg/kg body weight. Cefquinome concentrations in serum were determined by microbiological assay technique using Micrococcus luteus (ATCC 9341) as test organism. Following intramuscular injection of cefquinome in sheep and goats, the disposition curves were best described by two-compartment open model in both sheep and goats. The pharmacokinetics of cefquinome did not differ significantly between sheep and goats; similar intramuscular dose rate of cefquinome should therefore be applicable to both species. On comparing the data of serum levels of repeated intramuscular injections with first intramuscular injection, it was revealed that repeated intramuscular injections of cefquinome have cumulative effect in both species sheep and goats. The in vitro serum protein-binding tendency was 15.65% in sheep and 14.42% in goats. The serum concentrations of cefquinome along 24 h after injection in this study were exceeding the MICs of different susceptible microorganisms responsible for serious disease problems. These findings indicate successful use of cefquinome in sheep and goats. Mohamed El-Hewaity, Amera Abd El Latif, Ahmed Soliman, and Mohamed Aboubakr Copyright © 2014 Mohamed El-Hewaity et al. All rights reserved. Phenotypical and Genotypical Properties of an Arcanobacterium pluranimalium Strain Isolated from a Juvenile Giraffe (Giraffa camelopardalis reticulata) Wed, 30 Apr 2014 07:32:54 +0000 The present study was designed to characterize phenotypically and genotypically an Arcanobacterium pluranimalium strain (A. pluranimalium 4868) following necropsy from a juvenile giraffe. The species identity could be confirmed by phenotypical investigations and by MALDI-TOF MS analysis, by sequencing the 16S rDNA, pluranimaliumlysin encoding gene pla, and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap with sequence similarities to A. pluranimalium reference strain DSM 13483T of 99.2%, 89.9%, and 99.1%, respectively. To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. pluranimalium strain isolated from a giraffe. Karin Risse, Karen Schlez, Tobias Eisenberg, Christina Geiger, Anna Balbutskaya, Osama Sammra, Christoph Lämmler, and Amir Abdulmawjood Copyright © 2014 Karin Risse et al. All rights reserved. Effect of Sweet Orange Fruit Waste Diets and Acidifier on Haematology and Serum Chemistry of Weanling Rabbits Sun, 06 Apr 2014 07:02:40 +0000 A total of thirty-five mixed breed (35) rabbits of average weight of 700 g aged 5-6 weeks were allocated to seven treatments in a completely randomised design to investigate the effect of sweet orange fruit waste (SOFW) and acidomix acidifier on haematology and serum chemistry. The diets were 0% SOFW, 10% SOFW with 0.5% acidomix, 10% SOFW with 0.7 acidomix, 15% SOFW with 0.5% acidifier, 15% SOFW with 0.7% acidifier, 20% SOFW with 0.5% acidifier, and 20% SOFW with 0.7% acidifier. Blood samples were analyzed for haemoglobin (hb) concentration, white blood cells (WBC), red blood cells (RBC), differential WBC count (lymphocyte, basophil, eosinophil, monocyte, and neutrophil), alanine amino transferase (ALT), alkaline phosphatase (ALP), aspartate amino transferase (AST), total protein, albumin, and globulin. There was no interaction between SOFW and acidifier for the haematological and most of the serum chemistry parameters but significant difference was observed in ALT; however the values were within the normal range. SOFW had no significant effect on all haematological and serum chemistry parameters. Acidomix had significant effect () on haemoglobin concentration; rabbits fed 0.5% acidomix diets had higher values which were within the normal range. It is therefore concluded that SOFW with acidifier up to 20% had no detrimental effect on serum chemistry and haematology. Oluremi Martha Daudu, Rahamatu Usman Sani, Iyetunde Ifeyori Adedibu, Lawrence Anebi Ademu, Gideon Shaibu Bawa, and Taiye Sunday Olugbemi Copyright © 2014 Oluremi Martha Daudu et al. All rights reserved. Disparities in Spatial Prevalence of Feline Retroviruses due to Data Aggregation: A Case of the Modifiable Areal Unit Problem Thu, 20 Feb 2014 00:00:00 +0000 The knowledge of the spatial distribution feline immunodeficiency virus and feline leukemia virus infections, which are untreatable, can inform on their risk factors and high-risk areas to enhance control. However, when spatial analysis involves aggregated spatial data, results may be influenced by the spatial scale of aggregation, an effect known as the modifiable areal unit problem (MAUP). In this study, area level risk factors for both infections in 28,914 cats tested with ELISA were investigated by multivariable spatial Poisson regression models along with MAUP effect on spatial clustering and cluster detection (for postal codes, counties, and states) by Moran’s test and spatial scan test, respectively. The study results indicate that the significance and magnitude of the association of risk factors with both infections varied with aggregation scale. Further more, Moran’s test only identified spatial clustering at postal code and county levels of aggregation. Similarly, the spatial scan test indicated that the number, size, and location of clusters varied over aggregation scales. In conclusion, the association between infection and area was influenced by the choice of spatial scale and indicates the importance of study design and data analysis with respect to specific research questions. Bimal K. Chhetri, Olaf Berke, David L. Pearl, and Dorothee Bienzle Copyright © 2014 Bimal K. Chhetri et al. All rights reserved. Further Studies on Arcanobacterium phocisimile: a Novel Species of Genus Arcanobacterium Thu, 06 Feb 2014 10:03:45 +0000 Arcanobacterium phocisimile, a newly described species with the type strain A. phocisimile  isolated from a vaginal swab of a harbour seal and four additional A. phocisimile strains also isolated from four harbour seals could reliably be identified by phenotypic properties, by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), and by sequencing the genomic targets 16S rDNA and 16S-23S rDNA intergenic spacer region and the genes rpoB and gap. The A. phocisimile strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of A. phocisimile remains unclear. However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approach might help to identify A. phocisimile in future. Osama Sammra, Anna Balbutskaya, Muaz Hijazin, Samy Nagib, Jörg Alber, Christoph Lämmler, Amir Abdulmawjood, Ellen Prenger-Berninghoff, Markus Timke, Markus Kostrzewa, and Ursula Siebert Copyright © 2014 Osama Sammra et al. All rights reserved. S100β Levels in CSF of Nonambulatory Dogs with Intervertebral Disk Disease Treated with Electroacupuncture Sun, 29 Dec 2013 11:46:42 +0000 The aim of the study was to investigate S100β levels in the cerebrospinal fluid of nonambulatory dogs with intervertebral disk disease treated with electroacupuncture: 10 dogs with thoracolumbar disk extrusion graded 3 to 5 (EA group) and 7 dogs without neurologic dysfunction (control group). All dogs regained ambulation. S100β was detected by Western blot analysis where EA group dogs were evaluated at two time points (M1 = before EA and M2 = when the dogs return ambulation) and at one time point from control group. In EA group dogs M1-S100β levels were significantly higher than in control group. EA group dogs were divided into subgroups A (—early motor recovery; days) and B (—late motor recovery; days). M1-S100β levels were similar between subgroups A and B. However, M2-S100β levels were significantly higher in subgroup B than in subgroup A. An elevated S100β levels were observed in dogs with late motor recovery. S100β may be associated with neuroplasticity following spinal cord injuries with intervertebral disk extrusion. Further studies with larger numbers of subjects and control group with affected dogs are necessary to investigate the relationship between neurotrophic factors and electroacupuncture stimulation. Ayne Murata Hayashi, Ana Carolina Brandão Campos Fonseca Pinto, Silvia Renata Gaido Cortopassi, Valdecir Marvulle, Jessica Ruivo Maximino, Gerson Chadi, and Julia Maria Matera Copyright © 2013 Ayne Murata Hayashi et al. All rights reserved. Profile of Some Trace Elements in the Liver of Camels, Sheep, and Goats in the Sudan Sat, 07 Dec 2013 11:56:12 +0000 One hundred camels (Camelus dromedaries) and fifty sheep and goats being adult, male, and apparently healthy field animals were studied to provide data regarding the normal values of some hepatic trace elements. Liver samples were collected during postmortem examination, digested, and analyzed for Cu, Zn, Fe, Co, and Mn using atomic absorption spectrophotometry. The results showed that the differences in mean liver concentrations of Cu, Zn, Fe, and Co between camels, sheep, and goats were statistically significant (). Hepatic Cu, Fe, and Co concentrations were higher in camels than in sheep and goats. All liver samples were adequate for Fe and Co, whereas only camel liver was adequate for Cu. In camels, hepatic Zn concentration was inadequately lower than that in sheep and goats. No difference in Mn concentration was detected between camels, sheep, and goats. All liver samples were inadequate compared to free-ranging herbivores. In camels, significant correlation (, value = 0.04) was detected between Zn and Co, whereas in sheep significant correlation (, value = 0.026) was detected between Zn and Mn. No significant correlation between trace elements was detected in goats. Ibrahim Abdullah Ibrahim, Ali Mahmoud Shamat, Mohammed Osman Hussien, and Abdel Rahim Mohammed El Hussein Copyright © 2013 Ibrahim Abdullah Ibrahim et al. All rights reserved. Escherichia fergusonii Associated with Pneumonia in a Beef Cow Mon, 02 Dec 2013 14:43:56 +0000 An adult Angus cow developed hyperthermia, prostration, and respiratory distress, dying 36 hours after the onset of clinical signs. The main finding during postmortem examination was a severe focally extensive pneumonia. Icterus and a chronic mastitis were also noticed. Histologic examination of the lungs detected fibrinonecrotic pneumonia, with large number of oat cells and intralesional Gram-negative bacterial colonies. Samples from lung lesions were collected, and a pure growth of Escherichia fergusonii was obtained. E. fergusonii is a member of Enterobacteriaceae, related to Escherichia coli and Salmonella sp. In veterinary medicine, E. fergusonii has been reported in calves and sheep with clinical cases suggestive of salmonellosis; in a horse and a goat with enteritis and septicemia; and in ostriches with fibrinonecrotic typhlitis. To our knowledge, this report represents the first description of E. fergusonii associated with an acute pneumonia in cattle. Guillermo M. Rimoldi and Robert B. Moeller Jr. Copyright © 2013 Guillermo M. Rimoldi and Robert B. Moeller. All rights reserved. A Limited Sampling, Simple, and Useful Method for Determination of Glomerular Filtration Rate in Cats by Using a New Accurate HPLC Method to Measure Iohexol Plasmatic Concentrations Tue, 12 Nov 2013 14:35:19 +0000 Glomerular filtration rate (GFR) is still a highly underutilized tool in cats because available methods are not easy to be performed in clinical practice. Iohexol (IOX) has been shown to be a useful and reliable marker of GFR both in animals and in humans. The aim of the present study was to develop a rapid and reliable method for measuring IOX in feline plasma and to evaluate the accuracy of limited sampling models to establish a low-cost and clinically suitable GFR test. IOX concentrations were determined by using a new HPLC-UV method. GFR was assessed as plasma clearance of IOX, which was calculated by dividing dose administered by area under the curve of plasmatic concentration versus time (AUC), and indexed to body weight (BW). Correlation and agreement analysis between the GFR values obtained by a seven-point clearance method and the GFR values determined by the application of simplified sample combinations indicated that the 3-blood sample clearance model (5, 30, and 60 min) was the best simplified method because it provided an accurate GFR value in only one hour. The reported method is a simple and accurate way of GFR determination, which may be easily used in a clinical setting. Meucci Valentina, Guidi Grazia, Melanie Pierre, Breghi Gloria, and Lippi Ilaria Copyright © 2013 Meucci Valentina et al. All rights reserved. Effects of Different Amounts of Supplemental Selenium and Vitamin E on the Incidence of Retained Placenta, Selenium, Malondialdehyde, and Thyronines Status in Cows Treated with Prostaglandin F2α for the Induction of Parturition Thu, 07 Nov 2013 14:04:46 +0000 The incidence of retained placenta (RP) in cows increases in cases of parturition induced by prostaglandin F2α. We analyzed the effects of different doses of supplemental selenium and vitamin E on the incidence of RP, blood selenium, plasma thyronines, and malondialdehyde concentration. Thirty-three clinically healthy, multiparous Holstein-Frisian cows were assigned to 3 groups and supplemented with a single intramuscular injection of sodium selenite (SS) and tocopherol acetate (TAc) between days 250 to 255 of gestation: control—unsupplemented; group A—10 mg SS + 400 mg TAc; group B—20 mg SS + 800 mg TAc. Parturition was induced using PGF2α not before day 275 of gestation. The RP incidence was reduced from 66.7% in the control to 38.2 and 30.8% in groups A and B, respectively. Blood selenium and glutathione peroxidase activity in treated groups were significantly higher compared to control, with no significant difference between groups A and B. Plasma malondialdehyde in group B was significantly lower than that in control and group A, while thyronines levels were not affected. Comparison of RP and non-RP cows, independently of supplement treatment, revealed higher blood selenium and glutathione peroxidase activity and lower MDA and thyroxine in non-RP animals, while triiodothyronine level did not differ. Ivan B. Jovanović, Miljan Veličković, Dragan Vuković, Svetlana Milanović, Olivera Valčić, and Dragan Gvozdić Copyright © 2013 Ivan B. Jovanović et al. All rights reserved. Effects of Essential Oils and Polyunsaturated Fatty Acids on Canine Skin Equivalents: Skin Lipid Assessment and Morphological Evaluation Wed, 06 Nov 2013 16:04:53 +0000 A canine skin equivalent model has been validated for the assessment of a topical formulation effects. Skin equivalents were developed from freshly isolated cutaneous canine fibroblasts and keratinocytes, after enzymatic digestion of skin samples () from different breeds. Fibroblasts were embedded into a collagen type I matrix, and keratinocytes were seeded onto its surface at air-liquid interface. Skin equivalents were supplemented with essential oils and polyunsaturated fatty acid formulation or with vehicle. Skin equivalents were histopathologically and ultrastructurally studied, and the three main lipid groups (free fatty acids, cholesterol, and ceramides) were analyzed. Results showed that the culture method developed resulted in significant improvements in cell retrieval and confluence. Treated samples presented a thicker epidermis with increased number of viable cell layers, a denser and compact stratum corneum, and a more continuous basal membrane. Regarding lipid profile, treated skin equivalents showed a significant increase in ceramide content () when compared to untreated () samples. Ultrastructural study evidenced a compact and well-organized stratum corneum in both treated and control skin equivalents. In conclusion, cell viability and ceramides increase, after lipid supplementation, are especially relevant for the treatment of skin barrier disruptions occurring in canine atopic dermatitis. S. Cerrato, L. Ramió-Lluch, D. Fondevila, D. Rodes, P. Brazis, and A. Puigdemont Copyright © 2013 S. Cerrato et al. All rights reserved. Reproductive Parameters of the Dogo Argentino Bitch Mon, 28 Oct 2013 15:11:52 +0000 The Dogo Argentino (DA) is the first and only breed from Argentina recognized worldwide. Although its morphologic features have been well established, its normal reproductive parameters are not clearly known. The aim of this study was to determine the main DA bitch reproductive parameters. One hundred and forty-nine surveys were obtained from breeders from Córdoba province, Argentina: one for each intact DA bitch from 1 to 14 years old. The DA bitch reached puberty at an average of 8.93 months. The mean duration of vulval bleeding found in this study was 11.11 days. The clinical signs characteristic for proestrous-estrous were vulval edema (89.93%), bleeding during the time of mating (32.21%), holding the tail to the side (95.30%), and docility during mating (85.91%). DA bitches had a whelping rate of 84%. Out of 299 pregnancies, 89.30% exhibited a normal parturition, 6.69% presented dystocia, 2.68% needed Cesarean section, and 1.34% aborted. In conclusion, the reproductive parameters of the DA bitch are similar to those identified for other large breeds. DA often showed a prolonged vulval bleeding longer than proestrus. Its high whelping rate, its low incidence of dystocia, and its good maternal ability define the DA as a good reproductive breed with normal reproductive functions. Marina Caffaratti, Griselda González, Nora Gorla, and Corina Guendulain Copyright © 2013 Marina Caffaratti et al. All rights reserved. Frequency and Clinical Epidemiology of Canine Monocytic Ehrlichiosis in Dogs Infested with Ticks from Sinaloa, Mexico Thu, 24 Oct 2013 09:31:08 +0000 Ehrlichia canis is a rickettsial intracellular obligate bacterial pathogen and agent of canine monocytic ehrlichiosis. The prevalence of this disease in veterinary medicine can vary depending on the diagnostic method used and the geographic location. One hundred and fifty-two canine blood samples from six veterinary clinics and two shelters from Sinaloa State (Mexico) were analyzed in this study. All animals were suspected of having Canine Monocytic Ehrlichiosis (CME). The diagnostic methods used were the ELISA (Snap4Dx, IDEXX) together with blood smear and platelet count. From all dogs blood samples analyzed, 74.3% were positive to E. canis by ELISA and 40.1% were positive by blood smear. The sensitivity and specificity observed in the ELISA test were 78.8% and 86.7%. In addition, thrombocytopenia was presented in 87.6% of positive dogs. The predominant clinical manifestations observed were fever, anorexia, depression, lethargy, and petechiae. Consequently, this is the first report in which the morulae were visualized in the blood samples, and E. canis-specific antibodies were detected in dogs from Sinaloa, Northwest of Mexico. Carolina Guadalupe Sosa-Gutierrez, Maria Teresa Quintero Martinez, Soila Maribel Gaxiola Camacho, Silvia Cota Guajardo, Maria D. Esteve-Gassent, and María-Guadalupe Gordillo-Pérez Copyright © 2013 Carolina Guadalupe Sosa-Gutierrez et al. All rights reserved. Whole Body Computed Tomography with Advanced Imaging Techniques: A Research Tool for Measuring Body Composition in Dogs Thu, 10 Oct 2013 15:09:40 +0000 The use of computed tomography (CT) to evaluate obesity in canines is limited. Traditional CT image analysis is cumbersome and uses prediction equations that require manual calculations. In order to overcome this, our study investigated the use of advanced image analysis software programs to determine body composition in dogs with an application to canine obesity research. Beagles and greyhounds were chosen for their differences in morphology and propensity to obesity. Whole body CT scans with regular intervals were performed on six beagles and six greyhounds that were subjected to a 28-day weight-gain protocol. The CT images obtained at days 0 and 28 were analyzed using software programs OsiriX, ImageJ, and AutoCAT. The CT scanning technique was able to differentiate bone, lean, and fat tissue in dogs and proved sensitive enough to detect increases in both lean and fat during weight gain over a short period. A significant difference in lean : fat ratio was observed between the two breeds on both days 0 and 28 (). Therefore, CT and advanced image analysis proved useful in the current study for the estimation of body composition in dogs and has the potential to be used in canine obesity research. Dharma Purushothaman, Barbara A. Vanselow, Shu-Biao Wu, Sarah Butler, and Wendy Yvonne Brown Copyright © 2013 Dharma Purushothaman et al. All rights reserved. Sternal Aspiration of Bone Marrow in Dogs: A Practical Approach for Canine Leishmaniasis Diagnosis and Monitoring Sat, 28 Sep 2013 16:33:41 +0000 Bone-marrow aspirate material is commonly considered as one of the most sensitive tissues for a reliable diagnosis of leishmaniasis. The procedure herein described may permit less experienced veterinarians to be familiar with a quick and safe assessment method for leishmaniasis diagnosis in their patients. Animals are positioned in right lateral recumbency, and the area corresponding to the second, third, or fourth sternebra is identified and aseptically prepared. A 18-gauge needle connected to a 10 mL syringe is driven through the skin, up to the bone wall, and firmly pushed forward while rotating. Entry into the sternebra’s cavity is clearly perceived by the fall of resistance offered by the cortex. Some 2,500 sternal bone-marrow samplings were safely and efficiently performed on 887 dogs of different breeds and aging from 6 months to 14 years, during eight years of clinical activity for routine diagnosis of canine leishmaniasis in pets or for the efficacy evaluation of anti-Leishmania immunobiologicals in dogs naturally exposed to parasite transmission. Most of the samples (1716) were from 387 dogs enrolled for anti-Leishmania vaccine studies. The safety of the method was particularly assessed on these dogs that as per study protocol were submitted to repeated bone-marrow aspirations (2–4 per year) in follow-up examinations. Rosa Paparcone, Eleonora Fiorentino, Silvia Cappiello, Manuela Gizzarelli, Luigi Gradoni, Gaetano Oliva, and Valentina Foglia Manzillo Copyright © 2013 Rosa Paparcone et al. All rights reserved. Isolation and Molecular Characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in Chickens in Sudan Wed, 18 Sep 2013 13:46:03 +0000 The current study described the isolation and molecular detection of Mycoplasma gallisepticum (Mg) and Mycoplasma synoviae from tracheal swabs of diseased birds showing signs of respiratory distress in selected commercial (layer and broiler) farms and from yolk and an open air of pens of vaccinated breeder flocks in Sudan. A number of 45 Mycoplasma isolates were recovered from chickens in Khartoum, Gezira, and Equatoria states in Sudan. Of these, eight Mg and three Ms isolates were identified using growth inhibition and rapid serum agglutination (RSA) tests. The conventional PCR technique was applied to amplify 140 bp and 720 bp DNA fragments for the Mg and Ms, respectively. This research confirmed vertical and horizontal transmission of Mg from breeder farms through detection of Mg in yolk of fertile eggs and an air of pens despite previous vaccination. PCR is considered a rapid, sensitive, and cheap method and it will improve the diagnosis of Mycoplasma in chickens. Khalda A. Khalifa, Egbal Sidahmed Abdelrahim, Magdi Badwi, and Amal M. Mohamed Copyright © 2013 Khalda A. Khalifa et al. All rights reserved. Impact of Seminal Chemical Elements on the Oxidative Balance in Bovine Seminal Plasma and Spermatozoa Mon, 09 Sep 2013 11:53:26 +0000 Mutual relationships between selected chemical elements (Na, K, Fe, Cu, Mg, and Zn), basic motility characteristics (motility and progressive motility), and markers of the oxidative balance (superoxide dismutase, catalase, glutathione, albumin, and malondialdehyde) were investigated in bovine seminal plasma and spermatozoa. Computer assisted sperm analysis was used to assess the motility parameters; mineral concentrations were determined by the voltammetric method and flame absorption spectrophotometry; antioxidants and malondialdehyde were evaluated by UV/VIS spectrophotometry. Concentrations of chemical elements in both seminal fractions were in the following descending order: Na > K > Zn > Mg > Fe > Cu. Higher amounts of all minerals and nonenzymatic antioxidants were detected in the seminal plasma (; ), while higher MDA concentration and activity of enzymatic antioxidants were recorded in the cell lysates (; ). Na, Fe, Cu, Mg, and Zn were positively correlated with the motility and antioxidant parameters (; ; ). Inversely, K exhibited the positive associations with malondialdehyde (). This study demonstrates that most chemical elements are integral components of bovine semen and are needed for the protection against oxidative stress development. Eva Tvrdá, Norbert Lukáč, Monika Schneidgenová, Jana Lukáčová, Csaba Szabó, Zofia Goc, Agnieszka Greń, and Peter Massányi Copyright © 2013 Eva Tvrdá et al. All rights reserved. Surgical Management of Penile and Preputial Neoplasms in Equine with Special Reference to Partial Phallectomy Sun, 08 Sep 2013 14:52:23 +0000 Penile and preputial neoplasia in horses occurs infrequently and represents diagnostic and therapeutic challenges. The present study was carried out on a total number of 21 equids (14 stallions and 7 donkeys) suffered from different penile and preputial neoplasia. Diagnosis of neoplasms was based up on history of the case, clinical examination as well as histopathological evaluation. Animals with penile and preputial neoplasms were underwent local excision and partial phallectomy with a slightly modified version of the techniques described by William’s. The diagnosed neoplasms were penile and preputial squamous cell carcinomas (SCCs; ); sarcoid (); a-fibrosarcoma; and a melanoma. Local excision was curative in all cases except 5 stallions with SCCs. These stallions had extensive damage of the glans penis, free part of the penis and the inner lamina of the internal fold of the prepuce, and they underwent a partial phallectomy with successful outcome. Follow-up information was obtained by visit and telephone inquiries. In conclusion, penile and preputial neoplasms are commonly encountered in elderly male horses and SCCs are the most common type affecting male external genitalia. Partial phallectomy is effective for management of equine neoplasia if they are confined to the glans and body of the penis and there is no proximal spread or involvement to regional lymph nodes. Awad Rizk, Esam Mosbah, Gamal Karrouf, and Mohamed Abou Alsoud Copyright © 2013 Awad Rizk et al. All rights reserved. Epidemiological Overview of African Swine Fever in Uganda (2001–2012) Mon, 12 Aug 2013 16:39:50 +0000 African swine fever (ASF) is a contagious viral disease, which can cause up to 100% mortality among domestic pigs. In Uganda there is paucity of information on the epidemiology of the disease, hence a study was carried out to elucidate the patterns of ASF outbreaks. Spatial and temporal analyses were performed with data collected monthly by the district veterinary officers (DVOs) and sent to the central administration at MAAIF from 2001 to 2012. Additionally, risk factors and the associated characteristics related to the disease were assessed based on semistructured questionnaires sent to the DVOs. A total of 388 ASF outbreaks were reported in 59 districts. Of these outbreaks, 201 (51.8%) were reported in districts adjacent to the national parks while 80 (20.6%) were adjacent to international borders. The number of reported ASF outbreaks changed over time and by geographical regions; however, no outbreak was reported in the North-Eastern region. ASF was ranked as second most important disease of pigs, and it occurred mostly during the dry season (). Pig movements due to trade (OR 15.5, CI 4.9–49.1) and restocking (OR 6.6, CI 2.5–17.3) were the major risk factors. ASF control strategies should focus on limiting pig movements in Uganda. David Kalenzi Atuhaire, Sylvester Ochwo, Mathias Afayoa, Frank Norbert Mwiine, Ikwap Kokas, Eugene Arinaitwe, Rose Anna Ademun-Okurut, Julius Boniface Okuni, Ann Nanteza, Christosom Ayebazibwe, Loyce Okedi, William Olaho-Mukani, and Lonzy Ojok Copyright © 2013 David Kalenzi Atuhaire et al. All rights reserved. A Targeted Survey for Scrapie in Jos Plateau State, Nigeria Wed, 31 Jul 2013 12:55:29 +0000 Scrapie, a disease of sheep and goats with a progressive course and fatal outcome, has not been identified in Nigeria. Anecdotal scrapie reports by livestock workers abound. Livestock diseases like scrapie form huddles in livestock economics of countries. For 8 months we surveyed for scrapie targeting emergency/casualty slaughter sheep and goats in Jos, Nigeria. We clinically examined 510 sheep and 608 goats of local breeds, aged from 12 months to 5 years. In total 31 (5.10%) goats and no sheep were clinically suspicious for scrapie. Caudal brainstem tissues of suspect animals collected postmortem were analyzed for the disease specific form of the prion protein, PrPSc, using Bio-Rad’s TeSeE ELISA rapid test kit. No sample was positive for scrapie. Fluorescent antibody test for rabies and H&E staining on samples were carried out for differential diagnosis. These showed no pathological lesions indicative for neurological disease. While our findings do not exclude the presence of scrapie in Jos, we demonstrate that targeted sampling of small ruminants for neuroinfectious disease is feasible in developing countries, pointing to the possibility of implementing such a monitoring scheme in Nigeria to prevent economic losses in small ruminant livestock as scrapie caveats from endemic countries have shown. O. O. Nwankiti, E. I. Ikeh, O. A. Arowolo, A. J. Nwankiti, M. O. Odugbo, and T. Seuberlich Copyright © 2013 O. O. Nwankiti et al. All rights reserved. Traumatic Reticuloperitonitis in Water Buffalo (Bubalus bubalis): Clinical Findings and the Associated Inflammatory Response Tue, 30 Jul 2013 12:30:03 +0000 The present study was carried out to describe the clinical picture of traumatic reticuloperitonitis (TRP) in water buffalo (Bubalus bubalis) and to evaluate the inflammatory and immunologic responses for this clinical condition. Twenty-two buffalo with acute local TRP were monitored in our study. Additionally, 10 clinically healthy buffalo were randomly selected and served as controls. Acute local TRP was initially diagnosed by clinical examination and confirmed by ultrasonographic (USG) examination and/or necropsy findings. Blood samples were collected from all examined buffalo to measure the respective levels of tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, IL-10 and interferon gamma (INF)-γ, serum amyloid A (SAA), C-reactive protein (CRP), haptoglobin (Hp), fibrinogen (Fb), and serum sialic acid (SSA). It was found that TNF-α, IL-1β, IL-6, IL-10, SAA, CRP, Hp, Fb, and SSA were significantly higher in buffalo with TRP than the controls. Our findings suggest that the examined immunologic variables were helpful in documenting the inflammatory response in buffalo with TRP. However, their diagnostic usefulness only becomes apparent when considered in tandem with the clinical findings for any given animal, its anamnesis, and a subsequent USG assessment. Due to the frequent complications of TRP, more accurate indicators of its occurrence and severity would be useful. Maged El-Ashker, Mohamed Salama, and Mohamed El-Boshy Copyright © 2013 Maged El-Ashker et al. All rights reserved. Seroprevalence of Leptospiral Antibodies in Canine Population in and around Namakkal Tue, 09 Jul 2013 09:27:06 +0000 Leptospirosis is a reemerging and a complex zoonotic bacterial disease, caused by pathogenic serovars of Leptospira interrogans. A total of 124 sera samples of dogs belonging to different categories like vaccinated, unvaccinated-semiowned, and stray dogs were subjected to sampling. Microscopic agglutination test (MAT) was conducted by using Leptospira culture. Out of 42 vaccinated dogs, 24 (57%) were positive to one or more serovars. Of the 24, 22 (52.3%), 11 (26.19%), 4 (9.5%), 1 (3%), and 2 (4.7%) were positive to icterohaemorrhagiae, canicola, pomona, grippotyphosa, and autumnalis, respectively. Of the 48 unvaccinated semiowned dogs, 10 (28.8%) showed positive agglutination to one or more serovars. Of the 10 samples, 7 (14.5%), 2 (4.1%), 3 (6.2%), 3 (6.2%), and 5 (10.2%) were positive to icterohaemorrhagiae, canicola, pomona, grippotyphosa, and autumnalis, respectively. Among the 34 stray dogs, 12 showed positive agglutination to one or more leptospiral antibodies. Of the 12 samples, 6 (17.6%) showed positive agglutination to icterohaemorrhagiae, 2 (5.8%) to canicola, 5 (14.7%) to pomona, 7 (20.5%) to grippotyphosa, and 5 (4.7%) to autumnalis. This study emphasized the changing trends in the epidemiology of leptospirosis with higher prevalence of serovar L. grippotyphosa in street dogs. N. R. Senthil, K. M. Palanivel, and R. Rishikesavan Copyright © 2013 N. R. Senthil et al. All rights reserved.