Cell-Type-Specific Effects of Silibinin on Vitamin D-Induced Differentiation of Acute Myeloid Leukemia Cells Are Associated with Differential Modulation of RXRα Levels

Figure 2

Carnosic acid and silibinin, alone and in combination with 1,25D, inhibit proliferation of HL60 and U937 cells. Cells were seeded at 4 × 10⁴ cells/mL (day 0) and treated with test agents, for 96 h, as described in legend to Figure 1. Following incubation, cells were counted using the trypan blue exclusion assay. The numbers of viable cells are presented as the means ± SE of at least 5 independent experiments. *𝑃<0.05; **𝑃<0.01, or ***𝑃<0.001, treatments versus control.

(a) HL60

(b) U937