PKCδ Regulates Translation Initiation through PKR and eIF2α in Response to Retinoic Acid in Acute Myeloid Leukemia Cells
Figure 9
mTOR signaling is involved in regulation of (Ser51) of eIF2α through PKC and PKR. (a) Inhibition of PI3K/Akt/mTOR signaling pathway by rapamycin (20 nM), a specific mTOR inhibitor, led to induction of phosphorylation eIF2α in NB4 cells. Rapamycin treatment results in induction of p-(Ser51) eIF2α and PKR and p(Thr446)-PKR in NB4 cells. (b) NB4 cells were pretreated with a specific PI3K inhibitor (LY294002, 20 μM) induced PKCδ and p-eIF2α levels. ATRA Equal amounts of total cell lysate were subjected to Western blot analysis to detect p-eIF2α, p-Akt, and PKCδ expression.
