Mycophenolic Acid Overcomes Imatinib and Nilotinib Resistance of Chronic Myeloid Leukemia Cells by Apoptosis or a Senescent-Like Cell Cycle Arrest
Figure 3
MPA induced SA-β-Galactosidase activity. K562-S, K562-R, and K562-RN cells (2.105/mL) were grown in the presence of vehicle only or MPA (3 μg/mL) for 3 days. An aliquot was washed in PBS and 105 cells/well were fixed in PFA and then incubated overnight in a 96-well plate in the presence of X-Gal (1 mg/mL) at 37°C as described in Section 2. The day after, the cells were washed once in PBS and SA-β-gal activity was detected by a blue cell staining visualized under an inverted microscope. Pictures were acquired and analyzed using the NIS Nikon software (a). SA-β-gal-positive cells were quantified by counting 102 cells on three separate fields for each condition. Results show the mean of five independent experiments (b).
