New Quantitative Method to Identify NPM1 Mutations in Acute Myeloid Leukaemia

Figure 1

The strategy proposed for identification of NPM1 mutations. We proceeded in a two-step strategy with a first screening by HRM (high-resolution melting) analysis and then identification and quantification by allele-specific oligonucleotide—(ASO)-RQ-PCR. MRD: monitoring residual disease.