New Quantitative Method to Identify NPM1 Mutations in Acute Myeloid Leukaemia
Figure 2
HRM analysis and RQ-PCR of NPM1 mutations. (a) HRM profiles of 3 patients (in duplicate) harbouring NPM1 mutations (two A and one B types) compared to 9 negative patients. (b) One example of NPM1-A monitoring residual disease by real-time PCR from RNA. The final results were expressed as NPM1m/ABL copy number ratios in percent. Analysis was performed on the LC480 Roche device.
