Combination Phototherapy with a Histone Deacetylase Inhibitor and a Potent DNA-Binding Bibenzimidazole: Effects in Haematological Cell Lines
Figure 7
HDACi enhance UVASens/UVA-induced accumulation of γH2AX foci in R100 cells. Cells were pretreated with 10 μM SAHA for 24 hours prior to a 1-hour treatment with 0.1 μM UVASens and UVA radiation of cells with 50 J/m2. One hour after irradiation, cells were fixed and stained for γH2AX, following which images were acquired using a Zeiss 510 Meta Confocal Microscope using 0.5 μm z-sectioning (a). Images were stacked and merged using Metamorph software for immunofluorescence visualisation of γH2AX foci (green) in nuclei (TO-PRO-3, blue). The average number of γH2AX foci was quantified (a), and total nuclear fluorescence was measured (c) using Image J analysis software. Mean ± standard deviations from a single experiment performed in duplicate are shown. Bar = 20 μm; 63x magnification. **, *** compared to untreated cells.
