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Molecular Biology International
Volume 2010 (2010), Article ID 373241, 4 pages
http://dx.doi.org/10.4061/2010/373241
Research Article

Tissue-Specific Expression of the Chicken Calpain2 Gene

1Institute of Poultry Sciences, Sichuan Animal Science Academy, Chengdu, Sichuan 610066, China
2Breeding Center, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan 625014, China
3Sichuan Dahen Poultry Breeding Company, Chengdu, Sichuan 610066, China

Received 18 January 2010; Revised 21 June 2010; Accepted 30 June 2010

Academic Editor: Emanuel E. Strehler

Copyright © 2010 Zeng-Rong Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We quantified chicken calpain 2 (CAPN2) expression in two Chinese chicken breeds (mountainous black-bone chicken breed [MB] and a commercial meat type chicken breed [S01]) to discern the tissue and ontogenic expression pattern and its effect on muscle metabolism. Real-time quantitative PCR assay was developed for accurate measurement of the CAPN2 mRNA expression in various tissues from chickens of different ages (0, 2, 4, 6, 8, 10, and 12 weeks). Results showed that the breast muscle and leg muscle tissues had the highest expression of CAPN2 compared to the other tissues from the same individual ( ). Overall, the CAPN2 mRNA level exhibited a “rise” developmental change in all tissues. The S01 chicken had a higher expression of the CAPN2 mRNA in all tissues than the MB chicken. Our results suggest that chicken CAPN2 expression may be related to chicken breeds and tissues.