Expression of a Gene Encoding 34.9 kDa PPE Antigen of Mycobacterium avium subsp. paratuberculosis in E. coli
Figure 2
Coomassie Brilliant Blue stained 15% SDS-PAGE showing expressed PPE protein. Lane M: Prestained protein molecular weight marker. Lane 1: Purified recombinant 37.1 kDa his tag recombinant PPE protein. Lane 2: Whole cell extract of E. coli M15 harbouring 37.1 kDa fusion PPE-6xhistag protein (IPTG induced). Lane 3: Whole cell extract of E. coli M15 harbouring 37.1 kDa fusion PPE-6xhistag protein (IPTG uninduced). Lane 4: Whole cell extract of E. coli M15.
