Glucose-6-Phosphate Dehydrogenase of Trypanosomatids: Characterization, Target Validation, and Drug Discovery

Figure 5

Genetic validation of G6PDH as a drug target in bloodstream form T. brucei. Cumulative growth of wild-type (WT) trypanosomes and two independent cell lines (RNAiG6PDH A and B). In the absence of RNAi induction (−Tet), WT and RNAi parasites grow at equal rates; in the presence of the RNAi inducer tetracycline (+Tet), WT cells grow at a normal rate, while trypanosomes of the RNAi-cell lines die after 48 h. (Figure created with data from [45].)