Cryptolepine-Induced Cell Death of Leishmania donovani Promastigotes Is Augmented by Inhibition of Autophagy
Figure 7
Effect of inhibition of autophagy by 3-MA, on CLP-induced cell death. (a) L. donovani promastigotes were treated with 0.2% DMSO, 20 μM CLP, 10 mM 3-MA and 10 mM 3-MA prior to treatment with CLP for 2 h and percentage of cell viability was measured by MTT assay. Data are represented as Mean ± SEM (). (b) Flow cytometric analysis using annexin V and PI in FL-1 versus FL-2 channels. The cells were subjected to different treatments as shown in the figure for 2 h and 6 h, respectively, as described in Section 2. The annexin V positive cells (bottom right quadrant) denote apoptotic population.
