5^′CAG and 5^′CTG Repeats Create Differential Impediment to the Progression of a Minimal Reconstituted T4 Replisome Depending on the Concentration of dNTPs

Table 1

Description of plasmids and oligonucleotides.

p-Empty	Plasmid that does not contain any repeat
p-5′GTT16	Plasmid that contains 16 repeats of 5′-AAC/5′-TTG
p-5′CTG17	Plasmid that contains 17 repeats of 5′-CAG/5′-GTC
p-5′CAG23	Plasmid that contains 23 repeats of 5′-CTG/5′-GAC
p821	5′-CTGGCTAACTAGAGAACCCACTGCTTACTGGC-3′
p1033	5′-GATCAGCGAGCTCTAGCATTTAGGTGACAC-3′
p1033/4ps	5′-G◊A◊T◊C◊AGCGAGCTCTAGCATTTAGGTGACAC-3′
(50T/4ps)/p867	5′-T◊T◊T◊T◊(T)46CGACTCACTATAGGGAGACCCAAGCTAATT-3′
	◊indicates the position of the four phosphorothioate linkages within the oligonucleotide

The upper part of the table describes the plasmids. The middle part of the table refers to the oligonucleotides and their sequences. The lower part of the table shows the hybridization positions of the oligonucleotides.