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Molecular Biology International
Volume 2011 (2011), Article ID 507346, 13 pages
http://dx.doi.org/10.4061/2011/507346
Research Article

Molecular Characterization and SNP Detection of CD14 Gene of Crossbred Cattle

1Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar, Pin-243122, India
2Project Directorate on Poultry, Rajendranagar, Hyderabad, India
3Animal Nutrition Division, Indian Veterinary Research Institute, Izatnagar, Pin-243122, India
4Dairy Cattle Breeding Division, National Dairy Research Institute, Karnal, Haryana, Pin-132001, India

Received 27 April 2011; Revised 19 July 2011; Accepted 19 July 2011

Academic Editor: Andrzej Kloczkowski

Copyright © 2011 Aruna Pal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

CD14 is an important molecule for innate immunity that can act against a wide range of pathogens. The present paper has characterized CD14 gene of crossbred (CB) cattle (Bos indicus×Bos taurus). Cloning and sequence analysis of CD14 cDNA revealed 1119 nucleotide long open reading frame encoding 373 amino acids protein and 20 amino acids signal peptide. CB cattle CD14 gene exhibited a high percentage of nucleotide identity (59.3–98.1%) with the corresponding mammalian homologs. Cattle and buffalo appear to have diverged from a common ancestor in phylogenetic analysis. 25 SNPs with 17 amino acid changes were newly reported and the site for mutational hot-spot was detected in CB cattle CD14 gene. Non-synonymous substitutions exceeding synonymous substitutions indicate the evolution of this protein through positive selection among domestic animals. Predicted protein structures obtained from deduced amino acid sequence indicated CB cattle CD14 molecule to be a receptor with horse shoe-shaped structure. The sites for LPS binding, LPS signalling, leucine-rich repeats, putative N-linked glycosylation, O-linked glycosylation, glycosyl phosphatidyl inositol anchor, disulphide bridges, alpha helix, beta strand, leucine rich nuclear export signal, leucine zipper and domain linker were predicted. Most of leucine and cysteine residues remain conserved across the species.