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Molecular Biology International
Volume 2011 (2011), Article ID 938435, 4 pages
http://dx.doi.org/10.4061/2011/938435
Research Article

Establishment of Methylation-Specific PCR for the Mouse p53 Gene

1Department of Radiation Biology and Health, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka Yahatanishi-ku, Kitakyushu 807-8555, Japan
2Department of Immunology and Parasitology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka Yahatanishi-ku, Kitakyushu 807-8555, Japan

Received 30 March 2011; Revised 8 September 2011; Accepted 19 September 2011

Academic Editor: George A. Calin

Copyright © 2011 Ryuji Okazaki et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Methylation-specific PCR (MSP) of the mouse p53 gene has not yet been reported. We searched the CpG islands, sequenced the bisulfited DNA, and designed PCR primers for methylation and unmethylation sites. DNA from a young mouse produced a strong PCR product with the unmethylated primer and a weaker band with the methylated primer. DNA from an old mouse produced bands of similar intensities with both primers. In radiation-induced tumors, DNA from an old mouse yielded similar bands with both types of primers. We suggest that MSP is a valuable technique for the epigenetic study of the mouse p53 gene.