Table 1: Summary of the localization patterns observed for TRIM22.

Localization Pattern Cell Type Epitope Tag Reference Cytoplasm Diffuse Diffuse Diffuse Diffuse with speckles/bodies Diffuse Diffuse Diffuse with speckles/bodies 293T COS7 HeLa HeLa HeLa PBMCs U2OS GFP or V5/His GFP or V5/His Endogenous GFP GFP or V5/His Endogenous GFP [40] [40] [40] [22] [40] [40] [22] Cytoplasm & Nucleus Nucleoplasmic, with nuclear bodies1 Diffuse throughout, or nuclear bodies2 Nucleoplasmic, with nuclear bodies Diffuse, with cytoplasmic bodies3 Nucleoplasmic with NB4 Nucleoplasmic, with nuclear bodies Nucleoplasmic and cytoplasmic Diffuse with speckles5-7 ABC28 HeLa HeLa HeLa MCF7 MCF7 T47D U2OS Endogenous EGFP Endogenous FLAG EGFP, EYFP, or FLAG Endogenous Endogenous Endogenous [30] [30] [30] [43] [30] [30] [30] [42] Nucleus Aggregates/bodies Aggregates/bodies Diffuse with speckles/bodies Diffuse with speckles/bodies Diffuse with bodies 293 COS7 HepG2 HepG2 MCF7 Myc Myc Endogenous Myc FLAG [41] [10] [13] [13] [20]

1Some colocalization with fibrillarin (Nucleoli). 2Pattern changes with cell cycle phase: (G0/G1: nuclear bodies; S-phase: nuclear speckles and cytoplasmic; mitosis: diffuse throughout cell). 3TRIM22 plasmid was coexpressed with Rhesus TRIM5α. 4Partial colocalization with Cajal bodies. 5Potential colocalization with calnexin (Endoplasmic reticulum). 6Localization was primarily cytoplasmic when cells were fixed with paraformaldehyde, or both cytoplasmic and nuclear when fixed with ice-cold methanol. 7Partial colocalization with the centrosome.