Table 1: Summary of the localization patterns observed for TRIM22.

LocalizationPatternCell TypeEpitope TagReference

CytoplasmDiffuse
Diffuse
Diffuse
Diffuse with speckles/bodies
Diffuse
Diffuse
Diffuse with speckles/bodies
293T
COS7
HeLa
HeLa
HeLa
PBMCs
U2OS
GFP or V5/His
GFP or V5/His
Endogenous
GFP
GFP or V5/His
Endogenous
GFP
[40]
[40]
[40]
[22]
[40]
[40]
[22]

Cytoplasm & NucleusNucleoplasmic, with nuclear bodies1
Diffuse throughout, or nuclear bodies2
Nucleoplasmic, with nuclear bodies
Diffuse, with cytoplasmic bodies3
Nucleoplasmic with NB4
Nucleoplasmic, with nuclear bodies
Nucleoplasmic and cytoplasmic
Diffuse with speckles5-7
ABC28
HeLa
HeLa
HeLa
MCF7
MCF7
T47D
U2OS
Endogenous
EGFP
Endogenous
FLAG
EGFP, EYFP, or FLAG
Endogenous
Endogenous
Endogenous
[30]
[30]
[30]
[43]
[30]
[30]
[30]
[42]

NucleusAggregates/bodies
Aggregates/bodies
Diffuse with speckles/bodies
Diffuse with speckles/bodies
Diffuse with bodies
293
COS7
HepG2
HepG2
MCF7
Myc
Myc
Endogenous
Myc
FLAG
[41]
[10]
[13]
[13]
[20]

1Some colocalization with fibrillarin (Nucleoli).
2Pattern changes with cell cycle phase: (G0/G1: nuclear bodies; S-phase: nuclear speckles and cytoplasmic; mitosis: diffuse throughout cell).
3TRIM22 plasmid was coexpressed with Rhesus TRIM5α.
4Partial colocalization with Cajal bodies.
5Potential colocalization with calnexin (Endoplasmic reticulum).
6Localization was primarily cytoplasmic when cells were fixed with paraformaldehyde, or both cytoplasmic and nuclear when fixed with ice-cold methanol.
7Partial colocalization with the centrosome.