Loss of RASSF1A confers resistance to taxol-mediated apoptosis. A matched pair of RASSF1A ± cells was generated by stably knocking down RASSF1A expression in UCI-107 ovarian cancer cells using a RASSF1A-specific shRNA. Knockdown of RASSF1A was confirmed by western blotting. Tubulin served as a loading control (a). The UCI-107 RASSF1A ± cells were grown to 50% confluency and then treated with 25 nM Taxol or vehicle control 48 hours and cell number determined (b). Data represent an average of triplicate experiments, * compared to parental or vector control cells. (c). The RASSF1A ± UCI-107 cells were treated with 25 nM Taxol for 22 hours and caspase activation measured as a readout for apoptosis using a luminescent caspase activation assay. Data represent the average of two assays performed in triplicate. *, statistically different from vector control cells treated with taxol, .
