The ability of Taxol to promote tubulin acetylation is dependent on RASSF1A. The UCI-107 RASSF1A ± matched pair was treated with Taxol for 48 hours, cell lysates prepared and equal amounts of protein subjected to western blotting using antibodies specific for total or acetylated tubulin. The relevant bands from the western blot were quantified and average data from three experiments expressed as a ratio of acetylated tubulin to total tubulin to give a fold change. Knockdown of RASSF1A resulted in an approximately 50% reduction in the relative acetylation of tubulin, .
