Synergistic reactivation of RASSF1A expression by RG108 and Zebularine. (a). RASSF1A negative A547 ovarian cancer cells were treated with DMSO, Zebularine, RG108 or Zebularine and RG108 in combination for 48 hours and surviving cells counted as a measure of toxicity. Treatment with either of the demethylating agents resulted in no significant difference in cell number. (b). A547 cells were treated with the indicated doses of RG108 and Zebularine alone or in combination for 48 hours and cell lysates prepared. Equal amounts of proteins were immunoprecipitated with an anti-RASSF1A antibody and the immunoprecipitates subjected to Western analysis for RASSF1A. Densitometric quantification of the bands is shown below the figure.
