Modulator of Apoptosis 1: A Highly Regulated RASSF1A-Interacting BH3-Like Protein
Figure 3
Tumor inhibiting potential of the RASSF1A/MOAP-1 tumor suppressor pathway. A classical xenograft assay was carried out. Male athymic nude mice were injected subcutaneously with transiently transfected HCT116 cells mixed with matrigel mix into the right and left flank areas. Tumor volumes were measured until day 35 and plotted. values for MOAP-1 versus vector (0.019); RASSF1A versus vector (0.0001); MOAP-1 versus RASSF1A (0.02), n = 12–14. Statistical analysis was evaluated by Student’s -test (two-tailed). Protein expression at the time of subcutaneous injection was confirmed by immunoblotting (data not shown). Protein expression in HCT116 cells can be detected up to 10 days post-transfection. However, at the end of experiment, we could not detect protein expression of HA-RASSF1A or Myc-MOAP-1 in the resulting tumors. We argue that the growth properties of HCT116 cells containing the indicated expression constructs were programmed within the first 7–10 days and continued on that program even though expression detection of the indicated genes was not possible. Please refer to [1] for more details on this issue.
