Prevention of Lysosomal Storage Diseases and Derivation of Mutant Stem Cell Lines by Preimplantation Genetic Diagnosis

Figure 1

Characterization of SZ-Hunter HESCs for the expression of undifferentiated cell-specific markers, karyotype, and pluripotent potential. (a) RT-PCR products for the undifferentiated gene-specific markers OCT4, SOX2, NANOG, and REX-1, using cDNA-specific primers, in undifferentiated SZ-Hunter HESCs at passage P17. (b) Karyotype analysis for SZ-Hunter cells by Giemsa staining. (c) A typical morphology of an undifferentiated SZ-Hunter HESC colony. (d) A cystic embryoid body (EB) established from SZ-Hunter HESCs, grown for 20 days in culture of suspension.