Dynamic Association between HIV-1 Gag and Membrane Domains
Figure 1
(a) Gag accumulates at the uropod surface. While it remains to be determined whether the first contact between virus-producing and target cells occurs right at the uropod or elsewhere during VS formation, virus-laden uropods do participate in VS formation as determined by concentration of uropod markers at the VS. (b) A working model for a mechanism by which Gag multimers associate with rearward actin flow that directs Gag to the uropod. NC-dependent Gag multimerization underneath the plasma membrane promotes association between Gag multimer and UDM. Of note, in contrast to lipid raft and TEM markers, UDM proteins appear to accumulate at assembly sites of wild-type Gag as well as those of a Gag mutant that multimerizes but fails to bud (GNL, unpublished data).
