GAPDH Pseudogenes and the Quantification of Feline Genomic DNA Equivalents

Table 1

Details of the TaqMan real-time PCR assays.

Gene	Oligo	Sequence (5′→ 3′)	Amplicon size (bp)	Final conc. (nM)
GAPDH^1	Forward	GCCATCAATGACCCCTTCAT	82	480
	Reverse	GCCGTGGAATTTGCCGT		480
	Probe	CTCAACTACATGGTCTACATGTTCCAGTATGATTCCA^2		160
ALB^3	Forward	GATGGCTGATTGCTGTGAGA	150	500
	Reverse	CCCAGGAACCTCTGTTCATT		500
	Probe	ATCCCGGCTTCGGTCAGCTG^2,4		200

1[10]; ²5′FAM/3′TAMRA; ³present study; ⁴HPLC purification.