The Influence of DNA Extraction Procedure and Primer Set on the Bacterial Community Analysis by Pyrosequencing of Barcoded 16S rRNA Gene Amplicons

Table 1

Mean number of assigned sequences and number bacterial genera detected by three different data bank alignments after filtering for low sequence occurrence¹ (𝑛=12).

DNA Extraction	Primer set	Sequences	Genera
Procedure I	8f-534r	5004 (±1409)^a	54 (13.1 ± 2.1)^A
	968f-1401r	2748 (±493)^a	54 (15.5 ± 2.6)^A
Procedure II	8f-534r	16596 (±1790)^b	55 (23.9 ± 2.7)^B
	968f-1401r	10257 (±1423)^b	43 (18.0 ± 1.8)^AB

∗Total and mean genera per sample.
¹Different superscripts within columns are significantly different (𝑃≤0.05; ANOVA, capital letters = Tamhane test).