The Influence of DNA Extraction Procedure and Primer Set on the Bacterial Community Analysis by Pyrosequencing of Barcoded 16S rRNA Gene Amplicons

Table 2

𝑃-values of a multifactorial ANOVA analysis of total sequence reads and assigned bacterial genera before and after removal of low number sequences (<5).

Factor	Total sequence reads		Removal of low number sequences
	Sequence number	Genera	Sequence number	Genera
Extraction procedure	0.000	0.003	0.000	0.008
Primer	0.000	0.014	0.000	0.171
Extraction × Primer	0.000	0.001	0.000	0.003