Molecular Characterization of Human Rotavirus from Children with Diarrhoeal Disease in Sokoto State, Nigeria

Table 1

List of VP7 primers used for VP7 (G) genotyping.

Primer designation	Sequences (5′-3′)	Region amplified	Amplicon size
Beg9	GGCTTTAAAAGAGAGAATTTCCGTCTGG	1–29
End9	GGTACACATCATACAATTCTAATCTAAG	1062–1036
RVG9	GGTACATCATACAATTCT	1062–1044
aAT8-69M (G8)	GTCACACCATTTGTAAATTCG	178–198	885 bp
aBT1-Wa (G1)	CAAGTACTCAAATCAATGATGG	314–335	749 bp
aCT2-DS-1 (G2)	CAATGATATTAACACATTTTCTGTG	411–435	652 bp
aDT4-ST-3 (G4)	CGTTTCTGGTGAGGAGTTG	480–498	583 bp
aET3-P (G3)	CGTTTGAAGAAGTTGCAACAG	689–709	374 bp
aFT9-W161 (G9)	CTAGATGTAACTACAACTAC	757–776	306 bp
G10-(G10)	ATGTCAGACTACARATACTGG	666–687	397 bp
G12-(G12)	CCGATGGACGTAACGTTGTA	548–567	515 bp

Source: Noguchi Memorial Institute for Medical Research (NMIMR).