Soluble Expression and Characterization of Biologically Active Bacillus anthracis Protective Antigen in Escherichia coli

Table 2

Primers used in this study.

Primers	5′ to 3′ sequence	Restriction enzymes
pPROEXHTa_F	AGAGAAGGATCCAGAAGTTAAACAGGAGA	BamHI
pPROEXHTa_R	CATCTCACTCGAGTTATCCTATCTCATAGCCT	XhoI
pQE30_F	GCGCAGGCCGGATCCGAAGTTAAA CAG	BamHI
pQE30_R	CCTAGAGGTACCTTATCC	KpnI
pET 32c_F	GAGAAGGATCCGAATGGAAGTTAAACAGGAGA	BamHI
pET32c_R	CATCTCAGTCGACTTATCCTATCTCATAGCCT	SalI

Sequence underlined represents restriction sites.