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Mediators of Inflammation
Volume 7 (1998), Issue 6, Pages 417-420
http://dx.doi.org/10.1080/09629359890802

Acidosis activates complement system in vitro

1Department of Neonatology, Charité, Virchow-Hospital, Humboldt-University Berlin, Berlin 13353, Germany
2Department of Nephrology, Charité, Virchow-Hospital, Humboldt-University Berlin, Berlin 13353, Germany

Copyright © 1998 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We investigated the in vitro effect of different form s of acidosis (pH 7.0) on the formation of anaphylatoxins C3a and C5a. Metabolic acidosis due to addition of hydrochloric acid (10 μ mol/ml blood) or lactic acid (5.5 μ mol/ml) to heparin blood (N=12) caused significant activation of C3a and C5a compared to control (both p=0.002). Respiratory acidosis activated C3a (p=0.007) and C5a (p=0.003) compared to normocapnic controls. Making blood samples with lactic acidosis hypocapnic resulted in a median pH of 7.37. In this respiratory compensated metabolic acidosis, C3a and C5a were not increased. These experiments show that acidosis itself and not lactate trigger for activation of complement components C3 and C5.