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Mediators of Inflammation
Volume 8 (1999), Issue 4-5, Pages 189-197
doi:10.1080/09629359990342
The Mechanisms of Action of Interleukin-1 on Rabbit Intestinal Epithelial Cells
1Department of Physiology, Faculty of Medicine, American University of Beirut, Beirut, Lebanon
2Division of Gastroenterology, Winthrop-University Hospital, Mineola, NY, USA
3Stony Brook School of Medicine, Stony Brook NY, USA
4Americ an University of Beirut, Department of Physiology, Faculty of Medicine, Beirut, Lebanon
Copyright © 1999 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Interleukin-1 (IL-1) is an inflammatory mediator that increases Cl- secretion in intestinal epithelial cells. To identify the signal transduction pathway(s) involved in IL-1's action, cells were treated with IL-1 and the levels of cyclooxygenase (COX) enzymes, prostaglandin E2 (PGE2) and phospholipase A2-activating protein (PLAP), and the activity of phospholipase A2 (PLA2) were measured. IL-1 caused concentrationand time-dependent increases in the levels of PLA2 activity, and/or in the levels of PLAP, COX-2 and PGE2. The IL-induced increase in PGE2 levels was biphasic, with the first peak due to the increase in PLAP levels, and the second peak due to the increase in COX-2 levels. This increase in PGE2 levels may provide a mechanism for acute and chronic inflammation in the intestine.