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Mediators of Inflammation
Volume 12 (2003), Issue 5, Pages 309-313
Simultaneous measurement of multiple Th1 and Th2 serum cytokines in psoriasis and correlation with disease severity
1Department of Dermatology and Cutaneous Surgery, University of Miami/Jackson Memorial Medical Center, Holtz Center, Room 2042, 1611 NW 12 Avenue, Miami 33136, FL, USA
2Department of Pathology, University of Miami/Jackson Memorial Medical Center, Holtz Center, Room 2042, 1611 NW 12 Avenue, Miami 33136, FL, USA
Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background: Psoriatic plaques have been shown to contain increased levels of proinflammatory cytokines. Serum levels of interleukin (IL)-6, IL-7, IL-8, and interferon (IFN)- have been reported elevated in psoriatic patients.
Aim: To evaluate serum cytokine profiles in psoriasis patients by improved enzyme-linked immunosorbent assay (ELISA) technique and to correlate these levels with disease severity.
Methods: We analyzed single serum samples from 10 patients with active untreated psoriasis, two patients with active treated psoriasis, and five healthy volunteers for major T helper type 1 and T helper type 2 cytokines using the LINCOplex ELISA multi-analyte detection system that permits simultaneous detection of multiple cytokines from a single sample. The disease severity, including erythema, induration, scale, and surface area, was assessed.
Results: IFN- was markedly elevated in all sera from psoriasis patients, 33.8±1.3 pg/ml (mean±standard error) versus 8±1.5 pg/ml for normal controls , and positively correlated with all indices of disease severity (Spearman ). IL-8 was also increased in psoriasis patients (24.4±1.8 pg/ml) versus normal controls (3.6±1.2 pg/ml) and positively correlated with the degree of erythema (Spearman ). Mean IL-12 levels were decreased in sera from psoriasis patients (8.5±1.2 pg/ml) compared with normal controls (42.2±5.3 pg/ml) . Also, serum IL-10 levels were below detection levels in psoriatics compared with controls (6.4±1.3 pg/ml).
Conclusions: This new ELISA system allowed rapid and reliable detection of numerous cytokines in single serum samples from patients with psoriasis. We observed that IFN- and IL-8 cytokines were elevated in psoriatics and correlated with parameters of disease severity while IL-10 and IL-12 were decreased.