Down-Regulation of Tristetraprolin Expression Results in Enhanced IL-12 and MIP-2 Production and Reduced MIP-3<mml:math id="E1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>α</mml:mi></mml:math> Synthesis in Activated Macrophages

Jalonen, Ulla; Nieminen, Riina; Vuolteenaho, Katriina; Kankaanranta, Hannu; Moilanen, Eeva

doi:https://doi.org/10.1155/MI/2006/40691

Mediators of Inflammation

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Research Communication | Open Access

Volume 2006 | Article ID 040691 | https://doi.org/10.1155/MI/2006/40691

Down-Regulation of Tristetraprolin Expression Results in Enhanced IL-12 and MIP-2 Production and Reduced MIP-3α Synthesis in Activated Macrophages

Ulla Jalonen,¹Riina Nieminen,¹Katriina Vuolteenaho,¹Hannu Kankaanranta,¹and Eeva Moilanen¹

Received10 Jul 2006

Accepted25 Sept 2006

Published12 Dec 2006

Abstract

In inflammation, the post-transcriptional regulation of transiently expressed genes provides a potential therapeutic target. Tristetraprolin (TTP) is of the factors regulating decay of cytokine mRNAs. The aim of the present study was to identify cytokines whose expression is regulated by TTP. We established a TTP knock-down cell line by expressing shRNA against TTP (shTTP cell line). A cytokine antibody array was used to measure cytokine production in macrophages exposed to lipopolysaccharide (LPS). Cytokines IL-6, IL-12, TNF-α, and MIP-2 (a homologue to human IL-8) were expressed at higher levels whereas MIP-3α was produced at lower levels in LPS-treated shTTP cells than in control cells suggesting that the expression of these cytokines is regulated by TTP. The present data provide IL-12, MIP-2, and MIP-3α as novel inflammatory cytokine targets for TTP-mediated mRNA decay and stress the role of TTP in the regulation of the inflammatory process.

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Copyright © 2006 Ulla Jalonen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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