Myeloperoxidase Content is a Marker of Systemic Inflammation in a Chronic Condition: The Example Given by the Periodontal Disease in Rats

Table 1

Leukocyte number in blood collected from untreated control and treated periodontal-diseased rats.

Group	Blood leukocytes (total cells/mm^3; Mean ± SEM)
Naive	9,462 ± 352
Control	10,575 ± 1,213
Celecoxib	9,237 ± 936
Morphine	11,612 ± 226

On the 11th day of tooth ligation, naive, drug-treated (celecoxib at 12 mg/kg/day and morphine at 1 mg/kg/day), and saline treated groups of animals were anesthetized with 23 mg/kg xylazine and 116 mg/kg ketamine hydrochloride, and blood samples were obtained by femoral vein bleeding. Whole blood cell counting (cells/mm³) was performed using a mixture of 20 𝜇L of blood samples + 380 𝜇L of Turk Blue in a Neubauer chamber under a light microscope (x 200-fold). Celecoxib and morphine were systemically administered for 3 consecutive days, from 3rd to 5th day after ligature placement as described in Materials and Methods. Statistical analysis: 𝑃> .05, one-way ANOVA followed by Student-Newman-Keuls test, 4–6 animals per group.