Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour Treatment
Figure 2
Adhesion of PMN to tumour cells. 2 DiI-labeled PMN were added to culture wells containing confluent dermal fibroblasts (fibroblasts), HMVEC (HMVEC), HT-29 (HT-29), HT-29 with 10 g/mL of mouse anti-human CD44 monoclonal antibody, 10 g/mL of mouse anti-human CD162 monoclonal antibody and 10 g/mL of mouse anti-human CD18 monoclonal antibody in the medium (HT-29 + AbCocktail), FaDu (FaDu), FaDu with the Ab Cocktail (FaDu + AbCocktail), and HMVEC previously treated with 10 U/mL of human recombinant IL-1 for 5 hours (HMVEC + IL-1B) and incubated at C for 30 minutes. After washing, adhered PMN were evaluated as described in the Methods Section. Bars represent the mean SEM of 7 independent experiments performed by sixtoplicate. Statistical significance was assessed using ANOVA test; and when compared with HMVEC group.
